Table 1 Brief overview of iPSC generation and characterization
Bank Name | Cell Sources | Reprogramming Methods | Characterization methods |
|---|---|---|---|
California Institute for Regenerative Medicine (CIRM) | Blood cells (1148) Fibroblasts (263) | Episomal vectors | Characterization is carried out by Coriell and FCDI |
Coriell Institute for Medical Research (Coriell) | Fibroblasts Blood cells | Retrovirus (40%) Sendai virus (30%) Episomal vectors (27%) Lentivirus (3%) | General: post-thaw viability, mycoplasma detection, identity match, karyotyping, sterility, IF analysis of pluripotency Specific: Sendai virus clearance, Alkaline Phosphatase analysis of pluripotency, 3-germ-layer EB/teratoma differentiation, loss of episomal plasmids |
Fujifilm Cellular Dynamics International (FCDI) | Blood cells (1148) Fibroblasts (263) | Episomal vectors | General: mycoplasma detection, identity match, karyotyping, sterility, pluripotency analysis, loss of episomal plasmids |
Center for iPS Cell Research and Application (CiRA) | PBMC Cord blood Dental pulp | Episomal vectors Retrovirus | CiRA characterizes clinical-grade iPSCs by: post-thaw viability, mycoplasma detection, identity match, karyotyping, sterility, Sendai virus clearance, flow/microarray analysis of pluripotency, virus screening, SNV/INDEL/CNV, endotoxin |
European Bank for induced pluripotent Stem Cells (EBiSC) | Fibroblasts (> 75%) | Sendai virus (> 80%) Episomal vectors Retrovirus Transposon Lentivirus mRNA | General: post-thaw viability, mycoplasma detection, identity match, karyotyping, Sendai virus clearance Specific: IF/flow cytometry /Pluri analysis of pluripotency, 3-germ-layer EB differentiation, virus screening, CNV, RNA-seq, exome seq, genotyping array, methylation array |
Human Induced Pluripotent Stem Cell Initiative (HipSci) | PBMC (30) Fibroblasts (805) | Sendai virus | General: post-thaw viability, mycoplasma detection, identity match, Sendai virus clearance, Pluri analysis of pluripotency, CNV Specific: RNA-seq, exome-seq, genotyping array, methylation array, expression array, whole genome-seq, mass spectrometry, cellular phenotyping |
Human Disease iPSC Consortium Resource Center (Taiwan Human Disease iPSC Consortium) | PBMC Fibroblasts | Sendai virus | General: post-thaw viability, mycoplasma, identity match, karyotyping, Sendai virus clearance, IF/flow cytometry /RT-PCR analysis of pluripotency, 3-germ-layer EB/teratoma differentiation, CNV, SNP genotyping |
Institute of Physical and Chemical Research (RIKEN) | PBMC Cord blood Skin | Sendai virus (40%) Retrovirus (30%) Episomal vectors (30%) | General: post-thaw viability, mycoplasma detection, identity match Specific: karyotyping, ability to differentiate into specific cell type |
Korean National Stem Cell Bank (KSCB) | Fibroblasts | Sendai virus mRNA | General: mycoplasma, identity match, karyotyping, Sendai virus clearance, IF/RT-PCR analysis of pluripotency, 3-germ-layer EB/teratoma differentiation |
WiCell Research Institute (WiCell) | Blood cells | Sendai virus (> 50%) Episomal vectors (> 25%) Lentivirus Retrovirus | General: post-thaw viability, mycoplasma detection, identity match, karyotyping, sterility, Specific: spectral karyotyping, FISH, SNP microarray |