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Table 1 Brief overview of iPSC generation and characterization

From: Human iPSC banking: barriers and opportunities

Bank Name Cell Sources Reprogramming Methods Characterization methods
California Institute for Regenerative Medicine (CIRM) Blood cells (1148)
Fibroblasts (263)
Episomal vectors Characterization is carried out by Coriell and FCDI
Coriell Institute for Medical Research (Coriell) Fibroblasts
Blood cells
Retrovirus (40%)
Sendai virus (30%)
Episomal vectors (27%)
Lentivirus (3%)
General: post-thaw viability, mycoplasma detection, identity match, karyotyping, sterility, IF analysis of pluripotency
Specific: Sendai virus clearance, Alkaline Phosphatase analysis of pluripotency, 3-germ-layer EB/teratoma differentiation, loss of episomal plasmids
Fujifilm Cellular Dynamics International (FCDI) Blood cells (1148)
Fibroblasts (263)
Episomal vectors General: mycoplasma detection, identity match, karyotyping, sterility, pluripotency analysis, loss of episomal plasmids
Center for iPS Cell Research and Application (CiRA) PBMC
Cord blood
Dental pulp
Episomal vectors
Retrovirus
CiRA characterizes clinical-grade iPSCs by: post-thaw viability, mycoplasma detection, identity match, karyotyping, sterility, Sendai virus clearance, flow/microarray analysis of pluripotency, virus screening, SNV/INDEL/CNV, endotoxin
European Bank for induced pluripotent Stem Cells (EBiSC) Fibroblasts (> 75%) Sendai virus (> 80%)
Episomal vectors
Retrovirus
Transposon
Lentivirus
mRNA
General: post-thaw viability, mycoplasma detection, identity match, karyotyping, Sendai virus clearance
Specific: IF/flow cytometry /Pluri analysis of pluripotency, 3-germ-layer EB differentiation, virus screening, CNV, RNA-seq, exome seq, genotyping array, methylation array
Human Induced Pluripotent Stem Cell Initiative (HipSci) PBMC (30)
Fibroblasts (805)
Sendai virus General: post-thaw viability, mycoplasma detection, identity match, Sendai virus clearance, Pluri analysis of pluripotency, CNV
Specific: RNA-seq, exome-seq, genotyping array, methylation array, expression array, whole genome-seq, mass spectrometry, cellular phenotyping
Human Disease iPSC Consortium Resource Center
(Taiwan Human Disease iPSC Consortium)
PBMC
Fibroblasts
Sendai virus General: post-thaw viability, mycoplasma, identity match, karyotyping, Sendai virus clearance, IF/flow cytometry /RT-PCR analysis of pluripotency, 3-germ-layer EB/teratoma differentiation, CNV, SNP genotyping
Institute of Physical and Chemical Research (RIKEN) PBMC
Cord blood
Skin
Sendai virus (40%)
Retrovirus (30%)
Episomal vectors (30%)
General: post-thaw viability, mycoplasma detection, identity match
Specific: karyotyping, ability to differentiate into specific cell type
Korean National Stem Cell Bank (KSCB) Fibroblasts Sendai virus
mRNA
General: mycoplasma, identity match, karyotyping, Sendai virus clearance, IF/RT-PCR analysis of pluripotency, 3-germ-layer EB/teratoma differentiation
WiCell Research Institute (WiCell) Blood cells Sendai virus (> 50%)
Episomal vectors (> 25%)
Lentivirus
Retrovirus
General: post-thaw viability, mycoplasma detection, identity match, karyotyping, sterility,
Specific: spectral karyotyping, FISH, SNP microarray
  1. Note 1: Reprogramming methods are compiled from currently available cell lines. Cells not open on shelf or at the status of generation are not included in the percentage counts
  2. Note 2: Owing that iPSC banks collected cells from numerous organizations/institutions, characterization items may differ from line to line whilst deposited at the same bank. To acquire more comprehensive characterization items, it is suggested to look directly online at cell catalog of the bank. Characterization items summarized here list out “General” inspections that each cell line must undergo, and “Specific” examinations” that only performed on selected or applied lines
  3. Immunofluorescence(IF)/ Single nucleotide variation (SNV)/Insertion and/or deletion (INDEL)/ Reverse transcription-PCR (RT-PCR)/Fluorescence in situ hybridization (FISH)/ Single nucleotide polymorphism (SNP)/ Embryoid body (EB)