Fig. 6
From: Yy1 regulates Senp1 contributing to AMPA receptor GluR1 expression following neuronal depolarization
Yy1 modulates Senp1 to promote GluR1 expression. (a) Senp1 overexpression overcomes the reduction of GluR1 expression following membrane depolarization. Primary cortical neurons were transfected (Tf) with Flag alone or Flag-Senp1 with or without KCl treatment. Whole cell extracts were prepared and subjected to immunoblot with anti-GluR1, anti-Flag, and anti-Actin antibodies. Actin is used as a loading control. (b) Depletion of Senp1 reduces GluR1 protein. Total proteins were extracted and the expression of Senp1 and GluR1 were detected by western blotting. (c) Senp1 alleviates the decrease of GluR1 caused by Yy1 depletion. Yy1 siRNA (siYy1) or scramble control (scr) siRNAs together with Flag alone (+Flag) or Flag-Senp1 (+Flag-Senp1) were transfected into cells. Whole cell lysates were prepared 48 h later and subjected to western blot with anti-GluR1, anti-Flag, anti-Yy1, and anti-Actin antibodies. Actin was used as a protein loading control