Fig. 6
Restraint stress-induced antinociception (SIA) is prevented by i.c.v. blockade of NPSR or by i.pag. blockade of NK1Rs or mGlu5Rs. a-c: Time courses of antinociceptive effects (expressed as % MPE) induced by a 30 min-restraint stress (horizontal bars) in mice pre-treated with vehicle or the antagonist of NPSRs ([tBu-D-Gly5] NPS, 10 nmol, i.c.v.), NK1Rs (L-703,606, 10 nmol, i.pag.) or mGlu5Rs (MPEP, 30 nmol, i.pag.) in the hot-plate test. (two-way ANOVA /post hoc Bonferroni test). d: The AUC of the antinociceptive effect in each treatment group (one-way ANOVA /post hoc Tukey test). The antagonist was i.c.v. or i.pag. administered immediately before restraint stress. The data presentation and statistics are the same as in Fig. 2. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the vehicle control group; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the Stress group