Fig. 5

Oct4 acted as an upstream protein of HK2 and is also involved in the metabolic reprogramming of lung cancer cells with Collagen XVII overexpression. a, In lung cancer cells with collagen XVII overexpression, knockdown of Oct4 downregualted the mRNA and protein expression of HK2. b, Decreased glycosis in collagen XVII overexpressed lung cancer cells was noted when Oct4 was knockdown. c, The glucose uptake and lactate level were decreased in collagen overexpressed lung cancer cells with Oct4 knockdown. d, Oct4 did not affect the oxidative phosphorylation in lung cancer cells with collagen XVII overexpression. e, ATP content and the mitotracker red percentage were not different between collagen XVII overexpressed cells with or without Oct4 knockdown. f, Decreased sphere formation was observed in collagen XVII overexpressed lung cancer cells with Oct4 knockdown. g, Collagen XVII overexpressed cells with Oct4 knockdown were more sensitive to cisplatin treatment. h, The capability of tumor initiation was decreased in collagen XVII overexpressed lung cancer cells with Oct4 knockdown