Fig. 3

Anti-Dectin-2 monoclonal antibodies inhibited Dermatophagoides pteronyssinus-stimulated T-helper 2 cell cytokine production in monocyte-derived DC/CD14⁻ PBMC co-culture, PBMCs were obtained from six patients with asthma with high level of anti-D. pteronyssinus IgE. Monocyte-derived DCs (MoDCs) and CD14⁻ PBMCs were prepared as described in the Materials and Methods section. D. pteronyssinus was added to the co-cultures of MoDCs and CD14⁻ PBMC at a final concentration of 1 (a) or 3 (b) μg/mL. Anti-Dectin-2 monoclonal antibody (MoAb) 6A4G7 or 17A1D10 was added at a final concentration of 0.3, 1, or 3 μg/mL. IgG2a, at a final concentration of 3 μg/mL, was used as an isotype control. After 6 days of co-culture, the supernatant was removed, and the cells were co-cultured with the same concentration of D. pteronyssinus and anti-Dectin-2 MoAbs for another 6 days. The supernatant was harvested, and IL-5 and IL-13 levels were measured by ELISA. Mann–Whitney U test was used to compare the percent inhibition of cytokine release between anti-Dectin-2 MoAb and IgG2a. * p < 0.05; ** p < 0.01; *** p < 0.005