Fig. 1

Chemoresistant IGROV1 sublines exhibit high migratory ability. IGROV1 cells (WT) resistant to 2 μM cisplatin (CP), and 2 μg/ml SR-T100 (SRT) were isolated. a Phase contrast images of parental and chemoresistant cells. Scale bars, 100 μm. b In vitro transwell migration assay. Representative photomicrographs of cells that penetrated a filter of pore size of 8 μm. Scale bars, 200 μm. c Migrated cells were counted in 15 random fields on the lower surface of the filters and expressed as ratio (fold) of migrated cells compared with WT. d Cells were seeded into silicon inserts with 10% FBS medium. Following cell adhesion, inserts were removed and incubated for 36 h. Phase images were captured every 12 h and wound spaces were analyzed using ImageJ. e Cellular migratory ability is presented as the percentage of wound closure. Each bar represents mean ± SEM from three independent experiments. *: significant difference between chemoresistant (CP, SRT) and parental (WT) cells. *: p < 0.05; **: p < 0.01; ***: p < 0.001 by Student’s t-test