Fig. 10

Inhibition of SOCE sensitize chemoresistant IGROV1 cells to chemotherapeutic agents. Cell number was counted to evaluate the effect of SOCE inhibitors on chemotherapeutic agents-mediated cytotoxicity in chemoresistant IGROV1 (CP, SRT) cells. Combination treatment of SOCE inhibitors (2 μΜ SKF-96365, 10 μΜ YM-58483, 0.1 μM 2-APB) and chemotherapeutic agents (2 μΜ cisplatin, 15 μg/ml SR-T100) for 48 h in (a) IGROV1-CP and (b) IGROV1-SRT cells. Nuclear staining by DAPI showed existing cells and cell number was quantitative analyzed using ImageJ. Each bar represents mean ± SEM from three independent experiments. *: significant difference between cells treated with or without SOCE inhibitors (SKF-96365, YM-58483, 2-APB). *: p < 0.05; **: p < 0.01 by Student’s t-test