Fig. 2

Characters of focal adhesion molecules in chemoresistant IGROV1 sublines. Immunofluorescence staining of FAK, paxillin, vinculin and talin focal adhesion molecules was performed after fixation of IGROV1 parental (WT) and chemoresistant (CP, SRT) cells. a Representative fluorescence images of IGROV1 cells overexpressing EGFP-tagged focal adhesion molecules (FAK, paxillin, vinculin, talin) captured under a total internal reflection fluorescence microscope (TIRFM). Scale bars, 40 μm. b-d Quantitative analysis of the (b) number, (c) size, and (d) intensity of focal adhesions. Each bar represents mean ± SEM from at least 20 cells. a.u., arbitrary unit. *: significant difference between chemoresistant (CP, SRT) and parental (WT) cells. ***: p < 0.001 by Student’s t-test