Fig. 1

KSHV genome, early lytic gene expression and K-Rta RNA FISH. RNA-seq reads from KSHV-infected PEL cells at 24 h post-reactivation. Reads are positioned above a KSHV ORF map. Sequence reads for PAN RNA and another lncRNA (T0.7) predominate at this time point. The genomic position of the other viral components of the engine (ORF50 and ORF57) are indicated. The lower panel depicts the ORF50 locus in an expanded view showing the position of the RNA-FISH probes which detect unspliced K-Rta mRNAs and are used to image sites of viral transcription. Numbering beneath the transcripts (70-74) indicates viral map position in kb