Fig. 6
Phosphorylated PHB at Y259 by c-Kit in the membrane raft upregulates β-catenin—ABCG2 signaling. a Analysis of β-catenin and ABCG2 protein expression in SKOV3 and SKOV3_c-Kit cells by western blotting. b SKOV3 cells were transfected with empty vector, wild-type pD-PHB-HA, or mutant PHB plasmid (pD-PHBY259F-HA) for 48 h. The levels of β-catenin and ABCG2 were analyzed by western blotting. c Transfection with empty vector or mutant PHB plasmid (pD-PHBY259F-HA) in SKOV3_c-Kit, SKOV3GL-G4 or KURAMOCHI cells for 48 h. The levels of β-catenin and ABCG2 were analyzed by western blotting. Blots are representative of three independent experiments