Fig. 3

Virus overlay protein binding assay (VOPBA) with SH-SY5Y, U-87 MG, CHME-3 and C6 cell membrane proteins. Membrane proteins from neural cells were resolved by SDS-PAGE and transferred to nitrocellulose membrane. DENV-3 was overlaid on to the nitrocellulose membrane. The interacting proteins were detected by using flavivirus group-specific monoclonal antibody (4G2). Panel-I: Coomassie Brilliant Blue stained gel image depicting various cell membrane proteins. Membrane proteins fractions from SH-SY5Y (M1), U-87 MG (M2), CHME-3 (M3) and C6 (M4) were resolved by 12% SDS-PAGE. Lane L indicates the protein molecular weight ladder. Panel-II: VOPBA of neural cell membrane proteins using DENV-3. Lane A, B, C and D depicts overlay on cell membrane protein of SH-SY5Y, U-87 MG, and CHME-3 respectively. Note the presence of ~ 31 and ~ 28 kDa bands (arrow) across the three neural cell lines exhibiting positive interaction and no band in C6 cell line (negative control)