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Table 1 SEQUEST search results LC-MS/MS analysis of DENV-3 binding protein on SH-SY5Y cell membrane

From: Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

Accession

Gene symbol

Description

Sequest Score

PSMs

Unique Peptides

MW [kDa]

A

62414289

VIM

vimentin

1.85

1

1

53.62

32189394

ATP5B

ATP synthase subunit beta, mitochondrial precursor

2.78

1

1

56.53

4501881

ACTA1

actin, alpha skeletal muscle

1.64

1

1

42.02

B

221307584

PHB2

prohibitin-2 isoform 1

25.53

9

8

33.276

5174447

GNB2L1

guanine nucleotide-binding protein subunit beta-2-like 1

21.85

8

5

35.055

4506663

RPL8

60S ribosomal protein L8

18.08

5

4

28.007

4507879

VDAC1

voltage-dependent anion-selective channel protein 1

17.83

7

4

30.754

17158044

RPS6

40S ribosomal protein S6

15.62

5

5

28.663

386869503

RPS3

40S ribosomal protein S3 isoform 2

14.90

7

3

28.468

4506661

RPL7A

60S ribosomal protein L7a

14.40

5

5

29.977

208879465

VDAC3

voltage-dependent anion-selective channel protein 3 isoform 2

8.02

3

1

30.77

4502709

CDK1

cyclin-dependent kinase 1 isoform 1

7.73

3

3

34.074

15431301

RPL7

60S ribosomal protein L7

6.28

2

2

29.207

C

156071459

SLC25A5

ADP/ATP translocase 2

282.03

107

15

32.83

4506725

RPS4X

40S ribosomal protein S4, X isoform X isoform

210.99

89

28

29.58

55749577

SLC25A4

ADP/ATP translocase 1

158.48

63

4

33.04

528281407

PHB

prohibitin isoform 1

85.93

33

15

29.79

208973244

YWHAZ

14–3-3 protein zeta/delta

77.24

32

14

27.73

4505753

PGAM1

phosphoglycerate mutase 1 isoform 1

61.48

20

15

28.79

316659409

ACTG1

actin, cytoplasmic 2

55.26

24

10

41.77

4507949

YWHAB

14–3-3 protein beta/alpha

46.59

19

5

28.07

5803227

YWHAQ

14–3-3 protein theta

43.68

20

9

27.75

21464101

YWHAG

14–3-3 protein gamma

38.78

17

8

28.29

  1. Proteins are arranged in descending order based on scores obtained (A) ~ 50 kDa protein (B) ~ 31 kDa protein (C) ~ 28 kDa. VIM, 53 kDa proteins, PHB2, 33 kDa protein and PHB2, 30 kDa proteins were identified as bona fide protein to serve as a DENV-3 receptor on SH-SY5Y cells. Bold fonts represent the proteins identified in this study for further characterization based on the results obtained in the algorithm