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Table 1 SEQUEST search results LC-MS/MS analysis of DENV-3 binding protein on SH-SY5Y cell membrane

From: Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

AccessionGene symbolDescriptionSequest ScorePSMsUnique PeptidesMW [kDa]
A
62414289VIMvimentin1.851153.62
32189394ATP5BATP synthase subunit beta, mitochondrial precursor2.781156.53
4501881ACTA1actin, alpha skeletal muscle1.641142.02
B
221307584PHB2prohibitin-2 isoform 125.539833.276
5174447GNB2L1guanine nucleotide-binding protein subunit beta-2-like 121.858535.055
4506663RPL860S ribosomal protein L818.085428.007
4507879VDAC1voltage-dependent anion-selective channel protein 117.837430.754
17158044RPS640S ribosomal protein S615.625528.663
386869503RPS340S ribosomal protein S3 isoform 214.907328.468
4506661RPL7A60S ribosomal protein L7a14.405529.977
208879465VDAC3voltage-dependent anion-selective channel protein 3 isoform 28.023130.77
4502709CDK1cyclin-dependent kinase 1 isoform 17.733334.074
15431301RPL760S ribosomal protein L76.282229.207
C
156071459SLC25A5ADP/ATP translocase 2282.031071532.83
4506725RPS4X40S ribosomal protein S4, X isoform X isoform210.99892829.58
55749577SLC25A4ADP/ATP translocase 1158.4863433.04
528281407PHBprohibitin isoform 185.93331529.79
208973244YWHAZ14–3-3 protein zeta/delta77.24321427.73
4505753PGAM1phosphoglycerate mutase 1 isoform 161.48201528.79
316659409ACTG1actin, cytoplasmic 255.26241041.77
4507949YWHAB14–3-3 protein beta/alpha46.5919528.07
5803227YWHAQ14–3-3 protein theta43.6820927.75
21464101YWHAG14–3-3 protein gamma38.7817828.29
  1. Proteins are arranged in descending order based on scores obtained (A) ~ 50 kDa protein (B) ~ 31 kDa protein (C) ~ 28 kDa. VIM, 53 kDa proteins, PHB2, 33 kDa protein and PHB2, 30 kDa proteins were identified as bona fide protein to serve as a DENV-3 receptor on SH-SY5Y cells. Bold fonts represent the proteins identified in this study for further characterization based on the results obtained in the algorithm