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Table 2 SEQUEST search results of LC-MS/MS analysis of DENV-3 binding protein on U-87 MG cell membrane

From: Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

Accession

Gene symbol

Description

Sequest Score

PSMs

Unique Peptides

MW [kDa]

A

4507879

VDAC1

voltage-dependent anion-selective channel protein 1

203.68

106

16

30.75

221307584

PHB2

prohibitin-2 isoform 1

113.01

42

15

33.28

4507651

TPM4

tropomyosin alpha-4 chain isoform Tpm4.2cy

86.45

32

12

28.50

296317337

VDAC2

voltage-dependent anion-selective channel protein 2 isoform 1

74.70

37

10

33.35

4502317

ATP6V1E1

V-type proton ATPase subunit E 1 isoform a

32.81

14

11

26.13

23238211

ARPC2

actin-related protein 2/3 complex subunit 2

31.23

12

10

34.31

94721252

VAPA

vesicle-associated membrane protein-associated protein A isoform 2

29.45

11

7

27.88

4503477

EEF1B2

elongation factor 1-beta

27.62

12

5

24.75

B

156071459

SLC25A5

ADP/ATP translocase 2

147.37

66

10

32.83

528281407

PHB

prohibitin isoform 1

125.86

53

14

29.79

208973244

YWHAZ

14–3-3 protein zeta/delta

124.19

72

14

27.73

156071462

SLC25A6

ADP/ATP translocase 3

111.96

51

6

32.85

5803227

YWHAQ

14–3-3 protein theta

109.82

49

13

27.75

4507949

YWHAB

14–3-3 protein beta/alpha

91.79

39

6

28.07

4505753

PGAM1

phosphoglycerate mutase 1 isoform 1

91.30

38

14

28.79

21464101

YWHAG

14–3-3 protein gamma

59.11

26

6

28.29

4503727

FKBP3

peptidyl-prolyl cis-trans isomerase FKBP3

58.55

24

14

25.16

15431297

RPL13

60S ribosomal protein L13 isoform 1

49.72

21

13

24.25

  1. Proteins are arranged in descending order based on scores obtained (A) ~ 31 kDa protein (B) ~ 28 kDa protein. PHB2, 33 kDa protein and PHB1 30 kDa protein was identified as a bona fide protein to serve as a DENV-3 receptor on U-87 MG cells. Bold fonts represent the proteins identified in this study for further characterization based on the results obtained in the algorithm