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Table 2 SEQUEST search results of LC-MS/MS analysis of DENV-3 binding protein on U-87 MG cell membrane

From: Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

AccessionGene symbolDescriptionSequest ScorePSMsUnique PeptidesMW [kDa]
A
4507879VDAC1voltage-dependent anion-selective channel protein 1203.681061630.75
221307584PHB2prohibitin-2 isoform 1113.01421533.28
4507651TPM4tropomyosin alpha-4 chain isoform Tpm4.2cy86.45321228.50
296317337VDAC2voltage-dependent anion-selective channel protein 2 isoform 174.70371033.35
4502317ATP6V1E1V-type proton ATPase subunit E 1 isoform a32.81141126.13
23238211ARPC2actin-related protein 2/3 complex subunit 231.23121034.31
94721252VAPAvesicle-associated membrane protein-associated protein A isoform 229.4511727.88
4503477EEF1B2elongation factor 1-beta27.6212524.75
B
156071459SLC25A5ADP/ATP translocase 2147.37661032.83
528281407PHBprohibitin isoform 1125.86531429.79
208973244YWHAZ14–3-3 protein zeta/delta124.19721427.73
156071462SLC25A6ADP/ATP translocase 3111.9651632.85
5803227YWHAQ14–3-3 protein theta109.82491327.75
4507949YWHAB14–3-3 protein beta/alpha91.7939628.07
4505753PGAM1phosphoglycerate mutase 1 isoform 191.30381428.79
21464101YWHAG14–3-3 protein gamma59.1126628.29
4503727FKBP3peptidyl-prolyl cis-trans isomerase FKBP358.55241425.16
15431297RPL1360S ribosomal protein L13 isoform 149.72211324.25
  1. Proteins are arranged in descending order based on scores obtained (A) ~ 31 kDa protein (B) ~ 28 kDa protein. PHB2, 33 kDa protein and PHB1 30 kDa protein was identified as a bona fide protein to serve as a DENV-3 receptor on U-87 MG cells. Bold fonts represent the proteins identified in this study for further characterization based on the results obtained in the algorithm