Fig. 2

Effect of rLZE3 on the activation of bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells were stimulated with rZE3 or rLZE3 (100 nM) with or without polymyxin B (25 μg/mL). PBS and lipopolysaccharide stimulations were used as controls. a Dendritic cells were harvested after 20 h of stimulation. CD40 and CD80 expression levels in a CD11c⁺ gated population were evaluated by flow cytometry. A representative experiment is shown. b The mean fluorescence intensity (MFI) of cells stimulated with PBS was defined as the basal expression level. Relative MFIs are shown. The data represent the mean ± SE of the mean from three independent experiments. c TNF-α, IL-6 and IL-12p40 levels in the supernatants after 20 h of stimulation were evaluated by ELISA kits. The data represent the mean ± SE of the mean from three independent experiments