Fig. 7
Silencing of ZNF322A was attributed to its induction of autophagy via protein phosphorylation. Silencing of ZNF322A transcriptionally regulates PIM3 to phosphorylate IRS1S1101 and further inhibits the PI3K/AKT/mTOR pathways, limiting access of lung cancer cell to glucose. Silencing of ZNF322A induces phosphorylation of HSP27S82 and leads to activation of the unfolded protein response (UPR). Glucose starvation and heat stress-elicited activation trigger autophagosome formation in ZNF322A-silenced A549 lung cancer cells