Fig. 4

Normal iPSC lines have the ability to differentiate into different germ layer cells. a Immunofluorescence of various markers of normal iPSC derived cells: retinal pigment epithelium (RPE) marker RPE65, neural progenitor cells (NPC) specific antibody nestin, cardiac specific marker α-actinin, hepatic cell-specific markers albumin (ALB), β-cells precursor marker pancreatic and duodenal homeobox 1 (PDX1), endothelial cell marker PECM1. b-g Quantification of flow cytometry for the percentage of cells expressing b RPE65, c nestin, d α-actinin, e ALB, f PDX1, g PECM1. h Normal iPSC-derived cells express granulosa cell marker FSHR. i Flow cytometry analysis of troponin-I positive cells at 20 days after cardiomyocyte differentiation. j Quantification of flow cytometry data showing percentage of troponin-I positive cells in various normal and disease iPSC, and embryonic stem cell (ESC) lines undergoing cardiac differentiation. Data are represented as mean ± SEM