Fig. 2From: PEDF promotes the repair of bone marrow endothelial cell injury and accelerates hematopoietic reconstruction after bone marrow transplantationPEDF improves endothelial permeability. a Detection of PEDF level in irradiation injured endothelial cells by western blot. b Flow cytometry detected endothelial cell surface marker CD144 expression after irradiation. c Cell immunofluorescence (× 100) analysis of CD144 expression. d Transwell assay analysis of endothelial cell permeability and the expression of occludin and ZO-1. e PEDF shRNA (sh6 and sh7) were used to knock down PEDF and check the occludin expression in injured endothelial cells after irradiation (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001)Back to article page