Fig. 3

Effect of hG-CSF gene therapy on apoptotic modulators: Bcl2 and Bax. a–c qRT-PCR validating the expression levels of Bcl2, Bax and Bcl2:Bax mRNA ratio respectively, 4 days post BCAO. d–f Western blot analysis of the translated mRNA into protein expression of Bcl2, Bax. g Ratio of Bcl2:Bax protein expression. Representative densitometry for respective Western blots is also shown. Statistical analysis was performed by One Way ANOVA, with Tukey as post hoc test and expressed as means ± S.E.M; *p < 0.05/**p < 0.01/***p < 0.001 versus Sham group while ^#p < 0.05 /^##p < 0.01/^###p < 0.001 versus AAV-CMV-GFP- treated group. (n = 6 animals/group)