Fig. 1

CircNOL10 is down-regulated in BC tissues and cell lines. a Heat map from GEO microarray database (GSE101123) revealed the top ten increased and decreased circRNAs in eight BC samples and three normal subjects. b The schematic diagram displaying that circNOL10 is back-spliced from NOL10 gene, ranging from the 6th exon to the 12th exon. The back-splice junction sites and RT-PCR product of circNOL10 were verified by Sanger sequencing and agarose gel electrophoresis, respectively. c RT-PCR was used to confirm the existence of circNOL10 and NOL10 from cDNA and gDNA in BC cells using the divergent and convergent primers, respectively. d The expression of circNOL10 in tumor tissues and adjacent normal tissues from 60 BC patients was tested by qRT-PCR. e The overall survival rate of patients with high or low circNOL10 expression was assessed via Kaplan–Meier survival analysis. f The expression of circNOL10 was measured in six BC cell lines and a normal breast epithelial cell line MCF-10A by qRT-PCR. g qRT-PCR analysis of circular and linear NOL10 expression in BT-549 and MDA-MB-231 cells with or without RNase R treatment. h qRT-PCR analysis was used to determine the effect of Actinomycin D on the levels of circNOL10 and linear NOL10 in BT-549 and MDA-MB-231 cells at indicated time points. **P < 0.01, ***P < 0.001