Fig. 9

CircNOL10 suppresses tumor growth in vivo. BT-549 cells infected with lenti-vector or lenti-circNOL10 were subcutaneously injected into nude mice. a The tumor volumes were monitored every 4 days. b At 27 days post-inoculation, mice were killed and tumor weights were measured. c IHC staining was applied to evaluate the protein expression of proliferation index Ki-67 in the excised tumors. Scale bar, 50 μm. d qRT-PCR analysis of circNOL10, miR-767-5p and SOCS2 in xenograft tumors. e Western blot analysis of SOCS2, E-cadherin and N-cadherin proteins in tumor masses. f The schematic diagram displays that circNOL10 sponges miR-767-5p to protect SOCS2 from miR-767-5p-induced degradation, thus inactivating JAK/STAT signaling and suppressing BC progression. *P < 0.05, **P < 0.01, **P < 0.01