Fig. 4

Hsp47 interacting with FKBP10 enhanced the proliferation phenotype. a Interaction between FKBP10 and Hsp47 in glioma cells was detected by immunoprecipitation-Western blot assay. b Cellular localization of FKBP10 and Hsp47 was detected by immunofluorescence staining. DAPI was used to stain nuclei (blue). Scale bar = 30 μM. c, d The expression level of PCNA mRNA, PCNA protein, AKT, p-AKT, CREB, p-CREB and cell proliferation in Hsp47 parental, scramble, siRNAs groups of U118MG and LN229 cells. e Cell proliferation tests in Hsp47 parental, scramble, siRNAs groups of U118MG and LN229 cells by CCK-8 assays. f Representative images of colony formation assays in Hsp47 parental, scramble, siRNAs groups of U118MG and LN229 cells. Statistical results of colony number. The data are represented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001