Fig. 6

Gut microbiota from Fut2^△IEC mice promotes the generation of LPC, aggravating colitis a Body weight change of mice in FMT-WT group and FMT-F group during the process. b DAI evaluations for the two groups. (C,D) The length of colons from each group of mice. e Representative H&E staining images of colonic sections. f Immunofluorescent images of colon tissues stained with ZO-1 (green), and occludin (red) in each group (Scale bar, 100 μm). g The proteins level of TNF-α, IL-1β in colon tissues. GAPDH was used as a loading control. (H) Fecal concentration of LPC of mice in each group. Data are expressed as mean ± SEM (n = 5 per group). The data come from three independent experiments. In all panels: *p < 0.05, **p < 0.01, ***p < 0.001. (LPC lysophosphatidylcholine, H&E hematoxylin and eosin)