Glutaminase isoforms expression switches microRNA levels and oxidative status in glioblastoma cells

Table 1 Selected microRNAs

Name	Forward primer (5′-3′)	Human chromosome	Gene name	Gene region	GLS hybridization	GLS2 hybridization
microRNA-21-5p	TAGCTTATCAGACTGATGTTGA	17q23.1	VMP1	UTR	330 and 949
microRNA-92a-3p	TATTGCACTTGTCCCGGCCTGT	13q31.3	LINCRNA48	Intron, UTR
microRNA-140-3p	TACCACAGGGTAGAACCACGG	16q22.1	WWP2	Intron
microRNA-146a-5p	TGAGAACTGAATTCCATGGGTT	5q34	PTTG1	UTR		175
microRNA-203a	GTGAAATGTTTAGGACCACTAG	14q32.33			93
microRNA-363-3p	AATTGCACGGTATCCATCTGTA	Xq26.2
microRNA-487b-5p	GTGGTTATCCCTGTCCTGTTCG	Xq26.2	AL132709.4	Intron
microRNA-663a	AGGCGGGGCGCCGCGGGACCGC	20p11.1				29
microRNA-762	GGGGCTGGGGCCGGGGCCGAGC	16p11.2	BCL7C	Exon	1817
microRNA-1246	AATGGATTTTTGGAGCAGG	2q31.1			566	45
microRNA-1260a	ATCCCACCTCTGCCACCA	14p24.3	NGB	Exon		383
microRNA-1274a	TTCAGGTCCCTGTTCAGGCGCC	5p13.1	PLCXD3	Intron

Second column shows the primers used for targets amplification. Human karyotypic location of respective microRNAs were obtained from mirbase database (http://www.mirbase.org/). MicroRNAs respective sequences are present in some genes: name and location were determined using CoGemIR database (http://cogemir.tigem.it/), and hybridization with GLS and GLS2 genes were obtained using miRanda database (http://cbio.mskcc.org/microrna_data/miRanda-aug2010.tar.gz), and RNAhybrid (http://bibiserv.techfak.uni-bielefeld.de/rnahybrid/). VMP1: Vacuole Membrane Protein 1; LINCRNA48: Long Intergenic Non-Protein Coding RNA 48; WWP2: WW domain containing E3 ubiquitin Protein ligase 2; PTTGA: Pituitary Tumour-Transforming Gene 1; BCL7C: BCL Tumour Suppressor 7 C; NGB: Neuroglobine; PLCXD3: Phosphatidylinositol specific phospholipase C X Domain containing 3

ISSN: 1423-0127