Fig. 2

Deletion of proline-rich motifs on IGPR-1 eliminates its binding with NEDD4: a Schematic of IGPR-1 and Δ57/IGPR-1. PPR motif, poly-proline-rich motif, TM, transmembrane domain. b Whole cell lysates from HEK-293 cells expressing empty vector (EV), IGPR-1-Myc or Δ57/IGPR-1-Myc were subjected to in vitro GST-pull down assay using GST-NEDD4-WW#4 followed by Western blot analysis using anti-Myc antibody. The same membrane was blotted with anti-GST antibody. c Whole cell lysates corresponding to IGPR-1-Myc or Δ57/IGPR-1-Myc expressed in HEK-293 cells and loading control, GAPDH. d Whole cell lysates from HEK-293 cells expressing IGPR-1-Myc or Δ57/IGPR-1-Myc were subjected to co-immunoprecipitation assay using anti-Myc antibody followed by Western blot analysis using anti-HA antibody. e Whole cell lysates (WCL) corresponding to IGPR-1-Myc, Δ57/IGPR-1-Myc, HA-NEDD4 expressed in HEK-293 cells and loading control, GAPDH