Fig. 5

SHP is responsible for neuronal death caused by LRRK2-GS astrocytes. A Primary neurons isolated from LRRK2-WT and -GS mice were treated with tunicamycin, and then the indicated protein levels were analyzed by WB. B LRRK2-WT and -GS neurons were layered on top of LRRK2-WT or si-SHP or myc-XBP1-K276R transfected LRRK2-GS astrocytes for 7 d in vitro. Immunofluorescence staining of co-cultures was performed with indicated antibodies; representative results are shown. Summary data showing the fluorescence intensities of MAP2 and SHP per field (n = 12). Scale bar, 50 μm. Data are presented as means ± SD of three independent experiments (*p < 0.05). C Organotypic slices from LRRK2-WT and-GS mice were transfected with si-SHP (200 nM) for 48 h and then treated with tunicamycin (0.2 μg/ml) for 48 h. Representative images (left) and summary data (right) showing MAP2 and TUNEL staining. Five fields of view per group of three independent experiments (n = 15). Scale bar, 100 μm. Data are means ± SD (*p < 0.05, **p < 0.01)