Fig. 6

The effect of hsa-miR-21-5p knockdown on NF-κB activation. The effect of hsa-miR-21-5p on NF-κB activation was studied during hsa-miR-21-5p knockdown and CHPV infection in human microglial cells. A Western Blot analysis showed restoration of pNF-κB/NF-κB expression post knockdown of hsa-miR-21-5p and CHPV infection in human microglial cells. B The graph of densitometry analysis of pNF-κB/NF-κB showed significant suppression of pNF-κB expression by 60 % in hsa-miR-21-5p + CHPV compared to CHPV, while in hsa-miR-21-5p alone it was downregulated 25 % compared to Cy3 (C).The expression of IL-6 during hsa-miR-21-5p knockdown and CHPV infection was checked using qPCR. The expression of IL-6 was normalized to the expression of GAPDH. The expression of IL-6 got supressed in hsa-miR-21-5p Knockdown by 55 % as compared to Cy3. In hsa-miR-21-5p over-expression + CHPV, IL-6 expression was 60 % higher compared to CHPV (D). The expression of TNF-α 24 h post CHPV infection, was checked using qPCR. The expression of TNF-α was normalized to the expression of RNU6B. The expression of TNF-α got decreased in hsa-miR-21-5p knockdown by 65 % as compared to scramble. In hsa-miR-21-5p over-expression + CHPV, pNF-κBp65 /NF-κBp65 expression was 50 % supressed compared to CHPV. The experiments were performed in triplicate (n = 3) and shown as SE ± mean. One-way ANOVA has been applied using by using GraphPad-prism 8 (ver 8.3.0) to calculate the p-values, where: *p < 0.05, **p < 0.01, and ***p < 0.001