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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: MicroRNA-138-1-3p sensitizes sorafenib to hepatocellular carcinoma by targeting PAK5 mediated β-catenin/ABCB1 signaling pathway

Fig. 6

PAK5 facilitated the nuclear translocation of β-catenin. A The nuclear and cytoplasmic fractionation were performed to test the cellular distribution of β-catenin and p-β-catenin in Hep3B and HepG2 cells after transfection with PAK5/Vector, PAK5/WT, PAK5/S573N and PAK5/K478M plasmids. B The nuclear and cytoplasmic fractionation were performed to test the cellular distribution of β-catenin and p-β-catenin in Hep3B-SR and HepG2-SR cells compared with their parental. C The nuclear and cytoplasmic fractionation conducted to test the cellular distribution of β-catenin and p-β-catenin in Hep3B-SR and HepG2-SR cells after PAK5 knockdown. D Immunofluorescence was employed to show the cellular distribution of β-catenin in Hep3B cells after transfection with PAK5/Vector, PAK5/WT, PAK5/S573N and PAK5/K478M plasmids. E Immunofluorescence was employed to indicate the cellular distribution of β-catenin in Hep3B-SR and HepG2-SR cells compared with their parental. F and G The cell viability and flow cytometric analysis were used to assess the influence of β-catenin on cell viability and apoptosis in Hep3B and HepG2 cells

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Journal of Biomedical Science

ISSN: 1423-0127