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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Influenza a virus NS1 resembles a TRAF3-interacting motif to target the RNA sensing-TRAF3-type I IFN axis and impair antiviral innate immunity

Fig. 3

IAV NS1 uses its effector domain to block the K63-linked ubiquitination of TRAF3 during RIG-I signaling. A HEK293T cells were co-transfected with HA-tagged Ubiquitin (Ub), Flag-TRAF3, and delta RIG-I (ΔRIG-I) together with different NS1 constructs as indicated. Cell lysates were first immunoprecipitated by anti-Flag antibody and then immunoblotted by anti-HA antibody (left panel) or anti-K63-linked ubiquitin antibody (right panel). B HEK293T cells were transfected with HA-tagged K63 Ub, Flag-TRAF3, and GST-RIG-I-CARD together with different NS1 constructs as indicated. A similar IP- WB analysis was used to determine the effect of NS1 and its mutants on the K63-linked ubiquitination of TRAF3 during RIG-I signaling (left panel). Expression of Flag-TRAF3, GST-RIG-I-CARD, or NS1 constructs was confirmed by Western blot analyses using anti-Myc, anti-GST, or anti-Flag antibody as indicated (right panel). * indicated the non-specific band recognized by anti-Myc antibody

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Journal of Biomedical Science

ISSN: 1423-0127