Fig. 8

Pro-fibrotic injury downregulates expression of Epas1 and Epo of pericytes through ALK5. a The relative mRNA expression of Tgfb1, Acta2, Hif1a, Epas1 and Epo in the kidneys at day 0 (normal kidney) and day 4 after UUO surgery (UUO kidney). n = 5 at each time point. b Schema illustrating the analysis of kidneys and pericytes in mice with daily vehicle (Veh) or ALK5i SB431542 (5 mg/kg/day) treatment after UUO surgery. c The relative mRNA expression of Acta2, Hif1a, Epas1 and Epo in the contralateral (CLK) and UUO kidneys of mice with daily Veh or ALK5i SB431542 treatment after UUO surgery according to the schema in b. n = 5 at each time point. d The relative mRNA expression of Acta2, Hif1a, Epas1 and Epo in Col1a1-GFP⁺ pericytes isolated from kidneys before (day 0, normal kidney pericytes) and after (day 4 UUO kidney myofibroblasts) UUO surgery. n = 4 at each time point. e The relative mRNA expression of Acta2, Hif1a, Epas1 and Epo of Col1a1-GFP⁺ myofibroblasts isolated from kidneys of mice with daily Veh or ALK5i SB431542 treatment at day 4 after UUO surgery according to the schema in b. n = 4 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test in a, d and e, and by one-way ANOVA with Tukey’s test in c