Table 1 Direct targeting of KRAS in cancer via CRISPR systems (in chronological order)
CRISPR system | Cancer type | Targeted molecules | Cell type | Animal model | Publication year | Reference |
|---|---|---|---|---|---|---|
CRISPR/Cas9 | Lung | KRAS G12D G12C | A549, H23 (lung) | BalbC/nude A549 Xenografts | 2018 | [104] |
CRISPR/SpCas9 and dCas9-KRAB | Lung | G12S | A549, H2228 | NCG mice | 2020 | [105] |
CRISPR/Cas9 | CRC and Pancreas | G12V and G12D | SW480 (CRC) SW620 (CRC) SNU407 (CRC) AS-PC1 (PDAC) | n/a | 2018 | [106] |
CRISPR/Cas9 | CRC | G12V G12D G13D | HT29, SW403, SW480, SW620, LS513, and LoVo | BalbC nude | 2018 | [107] |
CRISPR/Cas9 | CRC | Whole gene knockout | SW480 | BalbC nude | 2020 | [108] |
CRISPR/dCas9/HDAC1 | CRC and Lung | Whole gene knockout | HCT-116 (CRC), NCI-H358 (Lung) | n/a | 2021 | [109] |
CRISPR/Cas9 | Pancreas | Whole gene knockout | PDAC cells (PACO9 & PACO19) | Nude mice | 2017 | [110] |
CRISPR-Cas13a | Pancreas CRC Lung | G12D | AsPC-1, PANC-1, HPAF-II, T3M4, LS174T, SK-LU-1 | n/a | 2017 | [112] |
CRISPR/Cas9 | Pancreas | KRAS G12D | PDAC: Panc-1 and SUIT-2 (h) TB32047 (murine) | n/a | 2019 | [117] |
CRISPR/CasRX | Pancreas | KRAS G12D G12C | n/a | Balb/C nude mice Orthotopic mice | 2020 | [120] |
CRISPR/Cas9 | Pancreas | KRAS G12D | FC1199, FC1242, FC1245 A9312 A9993 | Orthotopic mice | 2021 | [122] |