Fig. 3

Elp1 deficiency inhibits cell viability and colony formation, and induces apoptosis in MEFs in response to IR. A, B Cell numbers and clonogenic assays for Ctrl and KO MEFs that had either been non-radiated (no IR) or exposed to 4 Gy of IR at D5. A Cell numbers were counted at the indicated time-points. B At D10, cells were stained with crystal violet (left panel), and cell viability was quantified (right panel). C Analysis of apoptosis in Ctrl and KO MEFs with or without IR treatment at D10. Cells are classified as “late apoptotic” (top right quadrant), “necrotic” (top left), “live” (bottom left), or “early apoptotic” (bottom right). Representative scatter plots (left panel) and quantitative analysis (right panel) of early apoptotic cells are shown. Results represent the average of three independent experiments. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01 compared to Ctrl cells using Student’s t-test