Fig. 5

ROR2 inhibits tumor growth in vivo. A ROR2 expression inhibits tumor growth. A375-Empty and A375-ROR2 cells were xenotransplanted in nude mice (6 per group) and tumor volume was monitored twice weekly and presented as a mean ± S.D. Photographs of dissected tumors are shown. Statistical significance was tested by a one-tailed Student’s T-Test (n = 3). *: p < 0.01, **: p < 0.001, ***: p < 0.0001, n = 3. n.s.: no significant. B Tumors from mice injected with A375-ROR2 cells presented a reduced weight. The bar graph shows the mean ± S.D of the tumor weight from mice injected with A375-Empty and A375-ROR2. Statistical significance was tested by a one-tailed Student’s T-Test. ***: p < 0.0001. C Tumors from A375-ROR2 mice present reduced levels of p-Akt and of proliferation markers. Western blot analysis in tumors derived from xenografted A375-Empty and A375-ROR2 cells. T1 to T4 represent four different tumors in each group. GAPDH and β-actin were used as loading controls. D Tumors expressing ROR2 lack Akt phosphorylation. Immunohistochemistry (IHC) staining was used to examine both ROR2 and p-Akt levels in paraffin-stained tumor sections. Representative images at 40× magnification are shown. E ROR2 inhibits cell proliferation in vivo. Representative IHC staining of proliferative nuclei with Ki67 in xenograft tumor sections (40× magnification)