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Table 2 Inhibitory effects of mono- and tricarboxylates on the [3H]VPA uptake in WT and MT ALS cell lines

From: Monocarboxylate transporter functions and neuroprotective effects of valproic acid in experimental models of amyotrophic lateral sclerosis

Compounds

Concentration

[3H]VPA uptake (% of control)

(mM)

WT(hSOD1WT)

MT (hSOD1G93A)

Control

 

100 ± 4

100 ± 6

+ VPA

1

78.3 ± 4.5**

78.4 ± 1.6***

 

10

35.9 ± 5.2***

37.4 ± 4.2***

+ Acetic acid

1

54.9 ± 6.7 ***

40.3 ± 5.0***

 

10

24.4 ± 3.0***

15.4 ± 1.4***

+ Salicylic acid

1

64.9 ± 2.0**

78.8 ± 8.6*

 

10

17.9 ± 1.5***

31.9 ± 1.6***

+ PBA

10

43.1 ± 2.2***

56.6 ± 3.0***

+ CHC

10

67.7 ± 9.0 **

74.6 ± 7.1**

+ PAH

1

71.0 ± 5.5**

58.7 ± 2.9**

 

10

75.8 ± 2.3**

63.5 ± 5.5**

+ Citric acid

10

113 ± 3

125 ± 13

  1. The VPA transport study was performed in the presence or absence of several transporter substrates and inhibitors at concentrations of 1–10 mM for 10 s at 37 °C under acidic pH (pH 6.0). The data represent mean ± SEM (n = 3–4). ***p < 0.001, **p < 0.01, and *p < 0.05 represent significant difference from the WT control. CHC α-cyano-4-hydroxycinnamate; PAH para-aminohippuric acid
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Journal of Biomedical Science

ISSN: 1423-0127