Estradiol-mediated inhibition of Sp1 decreases miR-3194-5p expression to enhance CD44 expression during lung cancer progression

Table 1 Positive regulation of miRNAs expression by Sp1

miRNA	Sequence	Fold change (shSp1/shScr)*	Sp1-bound position**
miR-324-3p	ACTGCCCCAGGTGCTGCTGG	− 16.61	− 595
miR-135-5p	TATGGCTTTTTATTCCTATGTGA	− 16.61	− 59
miR-223*	CGTGTATTTGACAAGCTGAGTT	− 10.68	− 1108
miR-3922-5p	TCAAGGCCAGAGGTCCCACAGCA	− 9.49	+ 5
miR-2116*	CCTCCCATGCCAAGAACTCCC	− 7.12	− 389
miR-187	TCGTGTCTTGTGTTGCAGCCGG	− 7.12	+ 179
miR-494-5p	AGGTTGTCCGTGTTGTCTTCTCT	− 6.33	− 322
miR-2116	GGTTCTTAGCATAGGAGGTCT	− 5.34	− 389
miR-4484	AAAAGGCGGGAGAAGCCCCA	− 4.75	− 1022
miR-22	AAGCTGCCAGTTGAAGAACTGT	− 4.71	− 741
miR-223	TGTCAGTTTGTCAAATACCCCA	− 4.61	− 1108
let-7i	TGAGGTAGTAGTTTGTGCTGTT	− 4.48	− 744
miR-21*	CAACACCAGTCGATGGGCTGT	− 2.61	− 363
miR-379	TGGTAGACTATGGAACGTAGG	− 2.51	− 531
miR-548Q-5p	GCTGGTGCAAAAGTAATGGCGG	− 2.37	− 1506
miR-629	TGGGTTTACGTTGGGAGAACT	− 2.36	− 1699
miR-129-3p	AAGCCCTTACCCCAAAAAGCAT	− 2.02	− 484
miR-1307	ACTCGGCGTGGCGTCGGTCGTG	− 1.96	+ 197
miR-22*	AGTTCTTCAGTGGCAAGCTTTA	− 1.82	− 741
miR-33b*	CAGTGCCTCGGCAGTGCAGCCC	− 1.60	− 1143
miR-3194	GGCCAGCCACCAGGAGGGCTG	− 1.58	− 1844
miR-4423-5p	AGTTGCCTTTTTGTTCCCATGC	− 1.50	− 1237
miR-4423-3p	ATAGGCACCAAAAAGCAACAA	− 1.44	− 1237
miR-218-5p	TTGTGCTTGATCTAACCATGT	− 1.37	− 1087
miR-3158	AAGGGCTTCCTCTCTGCAGGAC	− 1.30	− 1422
miR-182-5p	TTTGGCAATGGTAGAACTCACACT	− 1.28	− 1310
miR-182*	TGGTTCTAGACTTGCCAACTA	− 1.23	− 1310
miR-769-5p	TGAGACCTCTGGGTTCTGAGCT	− 1.23	− 924

The global levels of miRNAs in Sp1-overexpression A549 cells were determined by small RNA sequencing. The gene repertoire of Sp1 binding in A549 cells was determined by ChIP-Seq. The miRNA repertoire listed here includes miRNAs that can be directly positively regulated by Sp1
^*The fold change between shSp1 and shScr normalized values from small RNA-seq data
^** The position of Sp1-bound regions with respect to the miRNA TSS

ISSN: 1423-0127