Fig. 2

Effect of DBPR114 on phosphorylation of AURKs and histone H3 in human HCC cells. HA22T/VGH and Huh7 cells were pretreated with nocodazole for 16 h prior to DBPR114 treatment lasting 2 h at the indicated concentrations. VX680 was used as the positive control for AURKA and AURKB and histone H3 phosphorylation. At the end of drug treatment, cells were lysed, and the soluble protein was separated using electrophoresis on a sodium dodecyl sulfate and polyacrylamide gel and analyzed through Western blotting. Autoradiographs were scanned for densitometric analysis using Image J software. Quantitation of the protein band was determined through normalization with the internal control GAPDH. Each bar represents the average value of three experiments. ND: not detected. Representative gel images are presented from three independent experiments. *p < 0.05 vs. DMSO-treated cells, ^&p < 0.01 vs. DMSO-treated cells, ^#p < 0.001 vs. DMSO-treated cells, measured using nonparametric t test