Fig. 3

ChREBP regulates the LPA-induced expression of fibrotic factors in SV40 MES13 cells. A and B SV40 MES13 cells were transfected with a control siRNA (siCon) or ChREBP siRNA (siChREBP), and treated with 10 µM LPA for 3 h. The mRNA (A) and protein (B) levels of ChREBP, fibronectin, TGF-β, and IL-1β were analyzed by qRT-PCR (n = 5) and western blotting (n = 3), respectively. C and D The SV40 MES13 cells were co-transfected with vectors expressing ChREBP and Mlx (ChREBP/Mlx), or transfected with an empty pcDNA vector (Con), and treated with 10 µM LPA for 3 h. The mRNA (C) and protein (D) levels of ChREBP, fibronectin, TGF-β, and IL-1β were analyzed by qRT-PCR (n = 5) and western blotting (n = 3), respectively. The data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.005 vs. siCon-BSA or Con-BSA; ^#p < 0.05, ^##p < 0.01, ^###p < 0.005 vs. siCon-LPA or Con-LPA