Fig. 6
Characterizations of mAbs. S14-1195 (A), S11-667 (B), RBD319-537 (C), or S331-524 D were coated on 96-well plates (0.4 μg per well). Three-fold serial dilutions of mAb-S5(●), -S22 (■), -S30 (▲), -S33 (◆), -S42(▲), and control mouse IgG (x) were prepared for standard ELISA. The binding activity of mAbs to S and S fragments was evaluated using an ELISA reader. The results are presented as the means ± standard errors of the means from triplicate wells. E Alignment of SARS-CoV-2 S331-524 and SARS-CoV-1 S318-510. Identical amino acid residues are marked in yellow. Each chimeric expression vector was transfected into 293T cells for 48 h. The cells were harvested and stained with FITC-labeled anti-His and Alexa 647-labeled mAbs. F Representative gating strategy for transfected 293T cells. G Recognition of each chimeric protein by mAbs was determined using flow cytometry. His− cells: black line; His+ cells: red line