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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: MicroRNA-485-5p targets keratin 17 to regulate oral cancer stemness and chemoresistance via the integrin/FAK/Src/ERK/β-catenin pathway

Fig. 2

Silencing of KRT17 downregulates cancer stemness in invasive OSCC. A KRT17 protein expressions in different OSCC cell lines were assessed by immunoblotting. B KRT17 mRNA expressions in the spheres formed from C9IV3 or HSC3 cells were analyzed by comparisons to their parental non-sphere C9IV3 or HSC3 cells, respectively, using qPCR. C Top, Protein expressions of KRT17 in C9IV3 and HSC3 cells transfected with Control-siRNAs (siCon) or three specific KRT17-siRNA plasmids (siKRT17-1, -2, -3) were assessed by immunoblotting. Middle, sphere-forming abilities of C9IV3 and HSC3 cells transfected with siCon or siKRT17 (siKRT17-3). Bottom, quantitative analysis of the number of spheres formed. D ALDH1, CD44 and CD133 expressions in spheres enriched from C9IV3 or HSC3 cells versus non-sphere cells were determined by qPCR. E ALDH1, CD44 and CD133 expressions in the C9IV3 or HSC3 spheres and non-sphere cells that had been transfected with siCon or siKRT17 were determined by qPCR. F Expressions of EMT markers Snail, Slug and Vimentin in the same spheres or non-sphere cells as in D, E. were determined by qPCR. Data are presented as mean ± SD (*p < 0.05, **p < 0.01 and ***p < 0.001)

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Journal of Biomedical Science

ISSN: 1423-0127