Fig. 1

DUSP3-deficient cells have defective TJ and barrier function. A Confluent DelA-F13 cells (DUSP3 +/+) and F16R cells (DUSP3−/−) were subjected to immunofluorescence (IF) staining using anti-ZO-1 (40–2200) and anti-DUSP3 (sc-374161) antibodies or an anti-pan-cadherin (CDH) antibody. DAPI was used for nuclear staining. B Cell lysates collected from DelA cell lines with different DUSP3 status were subjected to immunoblot analyses using indicated antibodies. C Confluent DelA cells were subjected to permeability assays against FITC-Dextran. The Student t-tests were performed between individual DUSP3-deficient cell lines and the mean value of three DUSP3 +/+ cell lines. D Trans-epithelial electric resistance (TEER) was measured in DelA cells after reaching confluence. The Student t-tests were performed between any individual DUSP3-deficient cell lines and DUSP3 +/+ cell lines. The data presented were the mean and standard deviations (*p < 0.05; **p < 0.01) of the triplicated experiments (C and D)