Fig. 11

Effects of infection on activation of pyroptotic markers in HBMEC cells and of caspase inhibitor Z-VAD on infected HeLa cells. A Lysates from HeLa cells, transfected with control RNA and with DYNLT1 siRNA (indicated as #2), and treated for 24 h with Z-VAD, were subjected to SDS-PAGE and immunoblotting using a specific antibody for PARP-1. Antibodies against GAPDH were used to verify loading while antibodies against DYNLT1 were used to check dynein silencing. B Signal intensity was quantified by densitometric analysis and reported as fold changes of DYNLT1 siRNA samples compared to their controls. Values are mean ± SEM of at least three independent experiments. C Signal intensity was quantified by densitometric analysis and reported as fold changes of infected samples compared to non-infected cells. Values are the mean ± SEM of at least three independent experiments. *P < 0.05 **P < 0.01 ***P < 0.001. D Lysates from HBMEC cells were subjected to SDS-PAGE and analyzed by immunoblotting using specific antibodies for Gasdermin E N-terminal (GSDME) and Caspase-1. Antibodies against vinculin were used to verify loading. E Signal intensity was quantified by densitometric analysis and reported as fold changes of infected samples compared to non-infected cells. Values are the mean ± SEM of at least three independent experiments. *P < 0.05 **P < 0.01 ***P < 0.001