Fig. 3

A Representative laser scanning confocal microscopic images from the RVLM showing cells that were immunoreactive to a neuronal marker, neuron-specific nuclear protein (NeuN; red fluorescence), and were additionally stained positively for VEGFR2 (green fluorescence). White dotted box in low-power view of the medulla oblongata indicated the location for high-power magnification. Scale bars, 200 μm (low-power view); 20 μm (high-power view). NA, nucleus ambiguous. B, C Fold-changes relative to sham-controls of vegfr2 mRNA (B) or VEGF protein (C) level in the RVLM in mice 2, 12 or 24 h after administration of azoxymethane (AOM; 100 μg/g, i.p.). Values are mean ± SEM, n = 3 animals per group. *p < 0.05 versus corresponding sham-controls group in the Dunnett multiple-range test