Fig. 4

CLEC5A and TLR2 are critical in SARS-CoV-2-induced collagen deposition SARS-CoV-2-challenged mice were sacrificed to harvest lung at 5 days post-infection. a Lung sections were stained with H&E and Masson’s trichrome. 20× magnification. b Picro Sirius Red dye and captured the image under light microscopy (upper panel) and polarized light microscopy (lower panel) under 20× and 40× magnification. The scale bar is 200 μm in 20× magnification and 50 μm in 40× magnification. c The level of collagen deposition (Pico Sirius Red) was quantified using MetaMorph™ software and presented as area (μm²). d The pulmonary fibrosis level was evaluated using Modified Ashcroft Scale. Data are mean ± SEM. ***p < 0.001 (Student’s t-test). N = 3 of each group