Fig. 1

Deletion of Oxtr in CA2 excitatory neurons of the mouse hippocampus. A Doubled-labeled confocal immunofluorescent images showing the colocalization of OXTRs (green) and STEP (red) in dCA2 of the mouse hippocampus. The inserts represent high magnification of the boxed area. Scale bar: 50 μm. SO stratum oriens, SP stratum pyramidale, SR stratum radiatum. B PCR screening of tail-derived genomic DNA for selection of Oxtr^−/− mice. C Dual-probe FISH images showing the expression of Oxtr mRNA and Amigo2 mRNA in dCA2 of WT and Oxtr^−/− mice (counterstained with DAPI, blue). Scale bar, 50 μm. Data was replicated in 4 mice. D Representative images with cresyl violet staining of dCA2 showing that the number of pyramidal neurons was not affected by targeted deletion of Oxtr compared with age-matched WT mice. Group data showing the summary results from 3–4 mice of each group at 12 weeks old. Scale bars: left, 500 μm; right, 50 μm. The total number of animal examined is indicated by n in parenthesis. Error bars represent the SEM