Fig. 7

Hesperetin treatment results in a younger transcriptome pattern. A A pie chart shows their biological processes and subcellular localization based on GO annotation. The GO was analyzed using the PANTHER functional classification. B Heatmap illustrating that a total of 141 DEGs are reversed by dietary hesperetin (126 up-regulated and 15 down-regulated genes; 26-month WT-Veh vs 3-month WT, FDR < 0.1) and the aged heart pattern is moved toward the pattern of a young heart. C The DEGs in the aged heart, which are reverted by hesperetin in panel (B), are grouped into different age-related pathways and presented as a percentage. D Heatmap illustrating that all 41 DEGs that are reversed by dietary hesperetin (9 up-regulated and 32 down-regulated genes; 26-month WT-Veh vs 3-month WT, FDR < 0.1) and the aged skeletal muscle (gastrocnemius); this showing a movement toward the pattern present in young skeletal muscle. E The DEGs in the aged skeletal muscles, which are reverted by hesperetin in panel (D), are grouped into different age-related pathways and presented by percentage. In this study, mice were treated with dietary hesperetin (100 mg/kg/day) or Veh control food from 21- to 26-month. The grouping of the pathways was carried out by MGI GO term finder (pathway p-value < 0.05)