Fig. 1

TRIM18 inhibits type I IFN production by human THP-1 macrophages in response to stimulations with dsRNA and dsDNA or infections with RNA and DNA viruses. a The immunoblot (IB) analysis of TRIM18, MAVS or STING expression in human THP-1 macrophages treated with shRNA to knockdown expression of TRIM18, MAVS or STING. A scrambled shRNA served as a control (sh-Ctrl) and the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as the loading control. The position of protein markers (shown in kDa) is indicated at right. b-h, ELISA of IFN-β production from human THP-1 macrophages treated with the indicated shRNA after a 12 h stimulations with dsRNA including 5’pppRNA (0.5 μg/ml) b and poly I:C (0.5 μg/ml) c or dsDNA from HSV-1 virus (HSV60, 2.5 μg/ml) d delivered by Lipofectamine 3000, or 12 h infections with RNA viruses including influenza A virus (influenza A virus PR8 strain, Flu PR8) e and Coxsackievirus B3 (CVB3) (f), or DNA viruses including HSV-1 g and adenovirus h at an MOI of 2 (n = 3 per group). Each circle represents an individual independent experiment and small solid black lines indicate the average of triplicates for results in (b–h). Mock, scrambled shRNA-treated cells without stimulation. NS, not significant (p > 0.05), **p < 0.01, ***p < 0.001, and p value was calculated by unpaired two-tailed Student’s t test. Data are representative of three independent experiments