Fig. 2

TRIM18 negatively regulates type I IFN production by BMDM upon stimulations of dsRNA and dsDNA or infections with RNA and DNA viruses. a–d ELISA of IFN-α a, c and IFN-β b, d production by BMDM from Trim18^+/+ (WT) and Trim18^−/− (KO) mice after 12 h of stimulations with 5’pppRNA (0.5 μg/ml), poly I:C (0.5 μg/ml) delivered by Lipofectamine 3000 a, b or infections with RNA viruses including influenza A virus (influenza A virus PR8 strain, Flu PR8) and Coxsackievirus B3 (CVB3) c, d at an MOI of 2 (n = 3 per group). e–h, ELISA of IFN-α e, g) and IFN-β f, h production by BMDM from Trim18^+/+ (WT) and Trim18^−/− (KO) mice after 12 h of stimulations with dsDNA from HSV-1 virus (HSV60, 2.5 μg/ml) and cGAMP (2.5 μg/ml) delivered by Lipofectamine 3000 e, f or infections with DNA viruses including adenovirus and HSV-1 g, h at an MOI of 2 (n = 3 per group). Each circle represents an individual independent experiment and small solid black lines indicate the average of triplicates for results. **p < 0.01, ***p < 0.001, and p value was calculated by unpaired two-tailed Student’s t test. Mock, wild-type BMDM without stimulation or infection. Data are representative of three independent experiments