Fig. 5
Salmonella + Alb-IL2 treatment enhance the effector function of T cells. BALB/c mice were inoculated with 5 × 105 CT26 tumor cells subcutaneously. 10 days later, mice were administered 50ug of Alb-IL2 intravenously. 1 day later, mice were treated with 5 × 106 Salmonella intravenously. Mice were treated again with 50ug of Alb-IL2 on day 17. Two weeks after the initiation of the treatment, tumor-infiltrating CD8 T cells were isolated and assessed for pro-inflammatory cytokine production. Representative flow gating and quantification of (a, b) CD8 + TNFα + , (c, d) CD8 + IFNγ + , and (e, f) CD8 + IL2 + for the indicated treatment groups. Similarly, CD8 T cells from the tumor draining lymph nodes were isolated and assessed. Representative flow gating and quantification of (g, h) CD8 + TNFα + , (i, j) CD8 + IFNγ + , and (k, l) CD8 + IL2 + in the tumor draining lymph nodes for the indicated treatment groups. N = 5 mice per group. Data is represented by mean ± SEM. P values were calculated by ordinary one-way ANOVA with the Tukey–Kramer multiple comparison test, and P < 0.05 is considered statistically significant. *,**,***, and **** indicate p values less than 0.05, 0.01, 0.001, and 0.0001, respectively