Fig. 1
From: Homozygous mutation of the LRRK2 ROC domain as a novel genetic model of parkinsonism
Detection of hLRRK2 and hLRRK2 R1441G expression and GTPase activity in Tg mice. A Real-time PCR assays of hLRRK2 and hLRRK2 R1441G mRNA expression in the brains of Tg mice. Data represent relative mRNA levels (mean ± SEM, n = 8) normalized to mouse apoB and are expressed in arbitrary units. B LRRK2 protein expression in the brains of non-Tg and Tg mice (n = 3). C hLRRK2 R1441G HEM and HOM mice lost less weight than non-Tg mice. Data are presented as the mean ± SEM (n = 6). D GTPase activity was measured using an enzyme-linked inorganic phosphate assay (ELIPA; n = 3). E Disruption of LRRK2 Ser935 phosphorylation in the brains of hLRRK2, R1441G HOM mice. Total lysates from the brains of 12-month-old hLRRK2 R1441G HOM mice were analyzed by western blotting for phosphorylation of LRRK2 at Ser935; actin was used as a loading control. *Significant effect, p < 0.05; **significant effect, p < 0.01; ***significant effect, p < 0.005; ****significant effect, p < 0.001