Fig. 6

MIC10 subcomplex is reduced when CYP11A1 complex is overexpressed. A Immunoblot detection of MIC10 and CYP11A1 complexes after separation of protein complexes from mitochondria of stable clones that overexpress CYP11A1 by blue-native gel electrophoresis. The names of the cells are written on top of each lane. Overexpression of CYP11A1 correlates with reduced MIC10 complex. B Quantification of band intensity for MIC10 and CYP11A1 complexes in CYP11A1-expressing C4 cells in arbitrary units (AU). Graph shows inverse relationship of CYP11A1 and MIC10 complexes. C Western blot of proteins separated by SDS-PAGE showing unchanged monomeric MIC10 amount in stable clones in the presence ( +) or absence (−) of doxycycline (doxy) induction