Fig. 2

Increased NETs formation in PBNs from DAH patients. a PBNs from SLE patients or HCs stimulated with PMA or LPS and stained with Sytox Green to detect DNAs morphology. Left, representative photographs from a HC and a SLE patient. Scale bar = 50 µm, magnification × 200. Right, quantification of NETs formation in HCs and SLE patients. b Quantification of NETs formation by PMA or LPS stimulation in DAH, LN and Nil patients. c CitH3 levels in PMA or LPS-stimulated supernatants from HCs and SLE patients. d CitH3 levels in PMA or LPS-stimulated supernatants from DAH, LN and Nil patients. Values are mean ± SEM. Horizontal lines are mean values. Patient numbers, n = 7 for SLE, n = 3 for DAH, n = 5 for LN, n = 5 for Nil. * p < 0.05, ** p < 0.01, *** p < 0.001